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You are here:Home » Molecular Biology » MB-Growth Factors-Interleukin » Interleukin 21, Recombinant, Human (IL-21)

Interleukin 21, Recombinant, Human (IL-21)

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Specifications

Recombinant protein corresponding tohuman IL-21, expressed in E. coli and purified via sequential chromatography.
Catalog #I8442-15
Biological ActivityED50 range = 1.0-5.0ng/ml (Specific activity: 1.0x10e6 - 2.0x10e5units/mg) determined by the dose-dependent
secretion of Interferon gamma by human natural killer lymphoma NK-92 cells. The optimal concentration for each specific application should be determined by an initial dose-response assay.
Amino Acid SequenceQDRHMIRMRQ LIDIVDQLKN YVNDLVPEFL PAPEDVETNC EWSAFSCFQK AQLKSANTGN NERIINVSIK KLKRKPPSTN AGRRQKHRLT CPSCDSYEKK PPKEFLERFK SLLQKMIHQH LSSRTHGSED S
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~15kD
SourceE. coli
Purity~95% (SDS-PAGE); Endotoxin: 0.1ng/ug
FormSupplied as a lyophilized powder. BSA free. Reconstitute with sterile ddH2O to a 0.01-1mg/ml. This solution can then be diluted with low endotoxin medium or a buffer with such as heat inactivated FCS or tissue culture grade BSA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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