Interleukin 21, Recombinant, Human (IL-21)
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| Interleukin-21 (IL-21), expressed by activated CD4(+) T cells, is the newest member of the common gamma-chain family of cytokines, which includes IL-2, IL-4, IL-7, IL-9, IL-13, and IL-15. Its private receptor, IL-21R, is expressed on T, B, and NK cells. Within the B cell lineage, IL-21 regulates IgG1 production and cooperates with IL-4 for the production of multiple Ab classes in vivo. IL-21R has been shown to activate the Janus kinase/signal transducers and activators of transcription signaling pathway upon ligand binding. Initial studies have demonstrated that IL-21 has pleiotropic effects on the proliferation, differentiation, and effector functions of B, T, natural killer, and dendritic cells. | | | Catalog # | I8442-15A | | Description | Recombinant Human IL-21 produced in E.Coli is a single, non-glycosilated polypeptide chain containing 133 amino acids and having a total molecular mass of 15,463 Dalton. The Recombinant Human IL-21 is purified by proprietary chromatographic techniques. | | Amino Acid Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Gly-Gln-Asp-Arg. | | Biological Activity | The ED50=1-10ng/ml as determined by the dose-dependent proliferation of human peripheral blood mononuclear cells is < 5ng/ml. | | Protein Content | Protein quantitation was carried out by two independent methods: | | 1. UV spectroscopy at 280 nm using the absorbency value of 0.54 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). | | 2. Analysis by RP-HPLC, using a standard solution of IL-21 as a Reference Standard. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O, 0.1% HSA or BSA, ,aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | | | Molecular Weight | 15.46kD | | Source | E. coli | | Purity | 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver stained gel. Endotoxin: 0.1ng/ug (IEU/ug) . | | Concentration | ~1mg/ml | | Form | Supplied as a lyophilized powder without additives. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml, which can then be further diluted to other aqueous solutions. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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