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You are here:Home » Molecular Biology » MB-Peptides » Interleukin 32 alpha, Recombinant, Human (IL-32a, Interleukin-32 Precursor, Natural Killer Cells Protein 4, NK4, TAIF, TAIFa, Tumor Necrosis Factor alpha-inducing Factor)

Interleukin 32 alpha, Recombinant, Human (IL-32a, Interleukin-32 Precursor, Natural Killer
Cells Protein 4, NK4, TAIF, TAIFa, Tumor Necrosis Factor alpha-inducing Factor)

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Specifications

Human IL-32 alpha is one of approximately 6 splice variants of a gene cloned from the human lung carcinoma stable transfectant, A549-Rbeta. IL-32 alpha has been shown to induce IL-8, TNF-a, and MIP-2 production from human & mouse macrophage cell lines. It is upregulated in activated T- & NK-cells, and IFN-g-treated epithelial cells.
Catalog #I8442-43
Biological ActivityHuman IL-32 alpha activity is measured via the dose-dependent induction of TNF-alpha production from human PBMCs. The ED50 is in the range of 0.101-152ng/ml.
AA SequenceMCFPKVLSDD MKKLKARMHQ AIERFYDKMQ NAESGRGQVM SSLAELEDDF KEGYLETVAA YYEEQHPELT PLLEKERDGL RCRGNRSPVP DVEDPATEEP GESFCDKSYG APRGDKEELT PQKCSEPQSS K
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile ddH2O containing 0.1% HSA or BSA. Reconstituted product is stable for 6 months at -20ºC. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight14.9kD
SourceRecombinant, human, produced in E. coli
Purity97% as determined by reducing and non-reducing SDS-PAGE.
FormSupplied as a lyophilized powder from 50mM sodium phosphate, pH 7.5. Reconstiute with sterile water containing 0.1% HSA or BSA to a concentration of 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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