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You are here:Home » Antibodies » Antibodies-Neurofilament » Anti -Internexin, alpha

Anti -Internexin, alpha


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Clone Host Grade Applications
Monoclonal Mouse Purified E B IH IF
Alpha-internexin is a, 66kD, type IV intermediate filament protein(1) that is expressed in neurons during development of the peripheral and central nervous systems, as well as in a few neurons of the adult central nervous system.(2) Recent studies have demonstrated that during neuronal development, alpha-internexin replaces type VI nestin and type III vimentin intermediate filament proteins.(3) Clinical studies have also demonstrated that alpha-internexin may play a role in the development of neuronal polarity.(4)
Catalog #I8442-74A
ApplicationsSuitable for use in ELISA, Western Blot, Immunoflourescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 0.1ug/ml
Western Blot: 0.1-0.5ug/ml
Immunohistochemistry (Formaldehyde fixed paraffin sections): 1-5ug/ml. Pretreat FFPE tissues with HIER using citrate buffer, pH 6.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
Clone No3G169
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified from mouse ascites fluid.
ImmunogenFull-length recombinant rat alpha-internexin protein
SpecificityRecognizes rat alpha-internexin at ~66kD. Species Crossreactivity: human, mouse and rabbit. Stains human neuron and nerve fiber in human cerebral cortex. Also stained glioma, ganglioneuroma, pheochromocytoma and paraganglioma in human tissue.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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