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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Internexin, alpha (NF66)

Anti -Internexin, alpha (NF66)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC FC
Neurofilaments (NF) are cytoplasmic intermediate filaments (IFs) found in neurons. NF-66, also known as -internexin, is a member of the IF family. Classified as a type IV IF, NF-66 is expressed by most neurons and has been shown to co-assemble with other NF triple proteins (light, medium and heavy NF) (1-2). In the adult brain, NF-66 is expressed at relatively low levels compared to the other NF proteins (3). NF-66 as also been shown to assemble into 10mm diameter IF in vitro.
Catalog #I8442-78B
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:25
Western Blot: 1:5,000-10,000
Immunohistochemistry: 1:250-500
Immunocytochemistry: 1:250-500
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k230
HostRabbit
SourceHuman
FormSupplied as a liquid.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues in the tail domain of human NF-66
SpecificityRecognizes human NF-66
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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