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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 22, Recombinant, Human (IL-22, CRF29, IL-TIF R1, IL-10 Related T Cell-derived Inducible Factor)

Interleukin 22, Recombinant, Human (IL-22, CRF29, IL-TIF R1, IL-10 Related T Cell-derived
Inducible Factor)

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Specifications

Human IL-22 Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179aa residue protein with a putative 33aa signal peptide that is cleaved to generate a 147aa mature protein that shares ~79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIFa and IL-TIFb, share > 98% sequence homology in their coding region. IL-22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10Ra (previously known as CRF2-4), belonging to the class II cytokine receptor family.
Catalog #I8443-13A
Molecular Mass The 147aa methionyl form of recombinant human IL-22 has a predicted Mr ~16.5kD.
AA Sequence MAPISSHCRL DKSNFQQPYI TNRTFMLAKE ASLADNNTDV RLIGEKLFHG VSMSERCYLM KQVLNFTLEE VLFPQSDRFQ PYMQEVVPFL ARLSNRLSTC HIEGDDLHIQ RNVQKLKDTV KKLGESGEIK AIGELDLLFM SLRNACI
Activity Measured by its ability to induce STAT3 activation in HepG2 cells. 10ng/ml can effectively induce STAT3 activation.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Stable for 12 months at -20°C or colder. Reconstitute to nominal volume by adding sterile buffer. Aliquot and store at -20°C or colder. Reconstituted product is stable for 3 months at -20°C or colder. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of Origin USA
SourceHuman DNA sequence encoding sequence (Ala34-Ile179), recombinant expressed in E. coli [(Dumoutier, L., et al., PNAS 97: 10144 (2000)].
Purity95% (SDS-PAGE and visualized by silver stain).
Endotoxin Level. 1EU/ug
FormSupplied as a lyophilized powder from PBS, 50ug of BSA per 1ug of cytokine.
ReconstitutionReconstitute with sterile PBS, 0.1% HSA or BSA to a concentration of > 10ug/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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