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Interleukin 22, Recombinant, Human (IL-22, CRF2–9, IL-TIF R1, IL-10 Related T Cell-derived Inducible Factor)
Interleukin 22, Recombinant, Human (IL-22, CRF2–9, IL-TIF R1, IL-10 Related T Cell-derived
Inducible Factor)
Pricing
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| Human IL-22 Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179aa residue protein with a putative 33aa signal peptide that is cleaved to generate a 147aa mature protein that shares ~79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIFa and IL-TIFb, share > 98% sequence homology in their coding region. IL-22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10Ra (previously known as CRF2-4), belonging to the class II cytokine receptor family. | | | Catalog # | I8443-13B | | Recombinant Human IL-22 is a single, non-glycosylated homodimeric polypeptide chain containing 2 x 146aa and having a total Mr 33,607D. | | Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Ile-Ser. | | Biological Activity | rHuIL-22 is fully biologically active when compared to standard. The Biological Acitivity was determined by the ability to activiate STAT following receptor ligand interaction. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. | | Source | Human, recombinant expressed in E. coli | | Purity | Chromatographically purified ( 97% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain). | | Dimers and Aggregates | < 1% (silver-stained SDS-PAGE gel analysis). | | Endotoxin | < 0.1ng/ug | | Concentration | ~0.1mg/ml | | Form | Supplied as a lyophilized powder in 40% mannitol, 30% HSA. | | Reconstitution | Reconstitute with sterile 18MO-cm H2O to a concentration > 100ug/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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