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Interleukin 22, Recombinant, Mouse (IL-22, IL-10 Related T cell-derived Inducible Factor, IL-TIF)
Interleukin 22, Recombinant, Mouse (IL-22, IL-10 Related T cell-derived Inducible Factor,
IL-TIF)
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| Mouse IL-22 Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with human IL-22 and IL-10, respectively. The mouse IL-22 gene is localized to chromosome 10. Although it exists as a single copy gene in many mouse strains, the IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIFa and IL-TIFb, share greater than 98% sequence homology in their coding region. IL-22 has been shown | | | to activate STAT-1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 produced by normal mouse T cells upon Con A activation. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10Rb (previously known as CRF2-4), belonging to the class II cytokine receptor family. | | | Catalog # | I8443-16 | | A DNA sequence encoding the IL-TIFa variant of mature mouse IL-22 protein sequence (Leu 34-Val 179) (Dumoutier, L. et al., 2000, J. Immunol. 164 | 1814-1819) was expressed in E coli. | | Molecular Mass | The 147 amino acid residue methionyl form of recombinant mouse IL-22 has a predicted molecular mass of approximately 16.5kD. | | Activity | Measured by its ability to induce IL-10 secretion in COLO 205 human colorectal adenocarcinoma cells. The ED50 for this effect is typically 60-300pg/ml. | | AA Sequence | MLPVNTRCKL EVSNFQQPYI VNRTFMLAKE ASLADNNTDV RLIGEKLFRG VSAKDQCYLM KQVLNFTLED VLLPQSDRFQ PYMQEVVPFL TKLSNQLSSC HISGDDQNIQ KNVRRLKETV KKLGESGEIK AIGELDLLFM SLRNACV | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.1% HSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 16.5kD | | Source | E coli | | Purity | 95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: 0.1 EU/ug (LAL). | | Form | Supplied as a lyophilized powder from PBS with 50ug of BSA per 1ug as a carrier protein. Reconstitute with sterile PBS, 0.1% HSA or BSA to 10ug/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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