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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 22 Binding Protein, Fc Chimera, Human (IL-22BP, Cytokine Receptor Family (CRF) 2-10, CRF2-X, IL-22 RA2)

Interleukin 22 Binding Protein, Fc Chimera, Human (IL-22BP, Cytokine Receptor Family (CRF)
2-10, CRF2-X, IL-22 RA2)

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Specifications

Human IL-22BP/Fc Chimera Interleukin 22 binding protein (IL-22BP), also known as cytokine receptor family (CRF) 2-10, CRF2-X, and IL-22 RA2, is a secreted glycoprotein belonging to the type II cytokine receptor family. The IL-22BP gene has been localized to chromosome 6 near the gene for IFN-£^ R1. It encodes a precursor protein of 231 amino acid (aa) residues with a 21 aa putative signal peptide and five potential N-linked glycolsylation sites. IL-22BP lacks a transmembrane and cytoplasmic domain and is most closely related to the extracellular domains of IL-22R (CRF2-9) and IL-20R (CRF2-8), sharing 33% and 34% aa sequence identity, respectively. It also shares sequence homology with the extracellular domains of IL-10R (29%), IL-10 Rb (30%), the IFN receptors (23-25%) and tissue factor (26%). IL-22BP antagonizes IL-22 activity by specifically binding IL-22 with high affinity and blocking its interaction with the cell surface IL-22 receptor heteromeric complex composed IL-22R and IL-20R. IL-22BP is expressed in multiple tissues. The
highest levels of expression are found in breast, lungs and colon.
Catalog #I8443-20
Source A DNA sequence encoding the signal peptide from human CD33 joined with the extracellular domain of human IL-22BP (Kotenko, S.V. et al., 2001, J. Immunol. 166:7096-7103) was fused to the Fc region of human IgG1 via a linker peptide. The chimeric protein was expressed in a mouse myeloma cell line, NS0. Human CD33 Signal peptide (Met 1-Ala 16) Human IL-22BP (Thr 22-Pro 231) IEGRMD Human IgG1 (Pro 100-Lys 330) N C
Molecular Mass he recombinant mature human IL-22BP/Fc chimera is a disulfide-linked homodimeric protein. Based on N-terminal amino acid sequencing, the mature protein starts at Thr 22 and has a calculated molecular mass of 51.3kD. As a result of glycosylation, the recombinant monomer migrates as an approximately 75-80kD protein in SDS-PAGE under reducing conditions.
Purity 90%, as determined by SDS-PAGE and visualized by silver stain.
Endotoxin 1.0 EU/ug of the cytokine as determined by the LAL method.
Activity Measured by its ability to bind rhIL-22. Immobilized rhIL-22BP/Fc at 10ug/ml (100ul/well) can bind rhIL-22 with a linear range of 12.5-0.8 ng/ml in a functional ELISA.
Form Lyophilized from a 0.2um filtered solution in PBS.
Reconstitution It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
Storage Lyophilized samples are stable for greater than six months at -20°C to -70°C. Upon reconstitution, this cytokine, in the presence of a carrier protein, should be stored under sterile conditions at -20°C to -70°C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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