Anti -Interleukin 24 (IL-24, C49A, FISP, IL10B, MDA-7, MDA7, MOB-5, ST16, Melanoma
Differentation-Associated Gene 7)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E IH FC |
|
| IL-24, also known as Melanoma Differentation-Associated gene 7 (MDA-7), is an IL-10 family cytokine produced by melanocytes and cells of the immune system. It functions to suppress tumor growth, induce apoptosis, and inhibit angiogenesis by signaling through the heterodimeric receptors IL-22 R alpha/IL-20 R beta and IL-20 R alpha/IL-20 R beta. | | | Catalog # | I8443-55 | | Applications | Suitable for use in Immunohistochemistry, Flow Cytometry and Direct ELISA. Other applications have not been tested. | | Recommended Dilutions | Immunohistochemistry: 25ug/ml detects IL-24 in human peripheral blood mononuclear cells. | | Flow Cytometry: 25ug/ml. 10ul labels 1-5x10e5 cells in a total reaction volume 200ul. For intracellular staining, cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. Following a 30 minute incubation with I8443-55, cells should be washed with 0.1% saponin prior to addition of a secondary developing reagent. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/ml solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1% saponin prior to flow cytometric analysis. | | Optimal dilutions to be determined by the researcher. | | Hybridoma | Fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, rhIL-24. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | N/A | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 8H135 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml (after reconstitution) | | Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Recombinant corresponding to human IL-24 expressed in NSO cells. | | Specificity | Recognizes human IL-24. In Western Blots, does not crossreact with recombinant mouse IL-24. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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