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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 27 (IL-27, Interleukin 17D, IL-17D)

Anti -Interleukin 27 (IL-27, Interleukin 17D, IL-17D)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH FC
IL-17D, also called IL-27, is one of six IL-17 family members. All are homodimers with conserved cysteines that form a cystine knot structure. IL-17D (Accession # AF479775) is a 52 kD dimer expressed by resting CD4 + T cells and CD19 + B cells that acts on endothelial cells to stimulate the release of IL-6, IL-8, and GM-CSF. With other IL-17 family members, IL-17D recruits neutrophils to sites of inflammation.
Catalog #I8443-75D
ApplicationsSuitable for use in Western Blot, Flow Cytometry, Immunohistochemistry and ELISA. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml. The detection limit is ~25ng/lane under non-reducing conditions. Reducing conditions are not recommended.
Flow Cytometry: 25ug/ml, 10ul labels 1-5x10e5 cells in a total reaction volume < 200ul. For intracellular staining, cells must be fixed and permeabilized using PBS, 4% paraformaldehyde, 0.1% saponin. Incubate for 30 minutes and wash cells with 0.1% saponin prior to addition of a secondary developing reagent. Cells should be washed a final time in 0.1% saponin prior to flow cytometric analysis.
Direct ELISA: 0.5-1ug/ml. Detection limit is ~5ng/well
Immunohistochemistry: 8-25ug/ml with appropriate secondary reagents to detect IL-17D in paraffin-embedded normal heart tissue sections.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG2b
Clone No7H53
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenE. coli-derived recombinant human Interleukin 27 (aa18-202)
SpecificityRecognizes human IL-27. Does not crossreact with recombinant human IL-17, IL17B, IL-17C, IL-17E or IL-17F. Species Sequence Homology: mouse, 86%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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