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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Involucrin

Anti -Involucrin

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH IF
Involucrin is expressed in a range of stratified squamous epithelia, including the cornea. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures it is expressed by all cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions and is reduced in severe dysplasias of the larynx and cervix.
Catalog #I8447-25
ApplicationsSuitable for use in ELISA (For coating, use antibody without BSA), Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunoprecipitation: 2ug/mg protein lysate (Denatured verified; Use Protein G).
Western Blot: 1-2ug/ml for 2 hours at RT.
Immunohistochemistry (Formalin/paraffin): 1:100-1:200 for 20 minutes at RT. For staining formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, for 10 minutes at 37°C or Protease XXV at 1mg/ml PBS, for 5 minutes at 37°C.
Note: Enzyme digestion is better than citrate buffer based epitope unmasking.
Optimal dilutions to be determined by the researcher.
Positive ControlLS174T cells and skin
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.341
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See I8447-25X.
PurityPurified by Protein G affinity chromatography.
ImmunogenInvolucrin from human keratinocytes. MW of Antigen
SpecificityRecognizes human involucrin at 66-170kD, depending on cell type. Stains involucrin in a variety of sizes
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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