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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Involucrin (IVL)

Anti -Involucrin (IVL)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant IH
Involucrin is a cytoplasmic protein and a precursor of the epidermal cornified envelope. It is expressed in a range of stratified squamous epithelia, including the cornea that lacks a distinct cornified layer. Involucrin may be a useful marker of terminal differentiation. In certain pathological conditions involucrin has been reported to have an altered expression. This antibody will help identify involucrin in a variety of tissues.
Catalog #I8447-25G
ApplicationsSuitable for use in Immunohistochemistry. Not recommended for use in Western Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:100-1:200 for 1 hr at RT. Trypsin digestion may be required.
Optimal dilutions to be determined by the researcher.
Positive Control
Normal human skin (cytoplasmic staining pattern)
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone NoSY5
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder, 15mM sodium azide. Reconstitute with 1ml sterile ddH2O.
PuritySupernatant
Immunogen120kD Human involucrin protein.
SpecificityRecognizes human Involucrin. Species Crossreactivity: Canine and swine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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