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You are here:Home » Antibodies » Antibodies-Extracellular Matrix Proteins » Anti -Involucrin (BSA & Azide Free)

Anti -Involucrin (BSA & Azide Free)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH IF
Involucrin is expressed in a range of stratified squamous epithelia, including the cornea. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures it is expressed by all cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
Catalog #I8447-25X
ApplicationsSuitable for use in ELISA, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunoprecipitation (Denatured verified): 2ug/mg protein lysate. Use Protein G.
Western Blot: 1-2ug/ml for 2 hours at RT.
Immunohistochemistry (Formalin/paraffin): 1:100-1:200 for 20 minutes at RT. For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 10 minutes at 37°C, or Protease XXV at 1mg/ml PBS for 5 minutes at 37°C. Enzyme digestion is more effective than citrate buffer-based epitope unmasking.
Optimal dilutions to be determined by the researcher.
Stains Involucrin As FollowsMCF-7 cells: 170kD
Gorilla, Owl monkey: Doublet of ~115kD and 150kD
Canine: 66kD
Porcine: 105kD Doublet
Positive ControlLS174T cells, Skin
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No0.N.341
FormSupplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See I8447-25.
PurityPurified by Protein G affinity chromatography.
ImmunogenInvolucrin from human keratinocytes.
EpitopeCodon 421-568.
Cellular LocalizationCytoplasmic
SpecificityRecognizes human Involucrin at 66-170kD, depending on cell type. Stains involucrin in a variety of sizes: 170kD in MCF-7 cells, a doublet of ~115kD and 150kD in gorilla and owl monkey, 66kD in canine and a doublet of 105kD in porcine.
Species CrossreactivityGorilla, owl monkey, porcine, canine and ra
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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