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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IP IH IF

Involucrin is expressed in a range of stratified squamous epithelia, including the cornea. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures it is expressed by all cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
Catalog #	I8447-25X
Applications	Suitable for use in ELISA, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Immunoprecipitation (Denatured verified): 2ug/mg protein lysate. Use Protein G.
	Western Blot: 1-2ug/ml for 2 hours at RT.
	Immunohistochemistry (Formalin/paraffin): 1:100-1:200 for 20 minutes at RT. For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 10 minutes at 37°C, or Protease XXV at 1mg/ml PBS for 5 minutes at 37°C. Enzyme digestion is more effective than citrate buffer-based epitope unmasking.
	Optimal dilutions to be determined by the researcher.
Stains Involucrin As Follows	MCF-7 cells: 170kD
	Gorilla, Owl monkey: Doublet of ~115kD and 150kD
	Canine: 66kD
	Porcine: 105kD Doublet
Positive Control	LS174T cells, Skin
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	0.N.341
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See I8447-25.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Involucrin from human keratinocytes. Epitope
Specificity	Recognizes human Involucrin at 66-170kD, depending on cell type. Stains involucrin in a variety of sizes
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.