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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP IH IC IF

Involucrin is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. The gene consists of 585aa, 390 of which form a central decapeptide repeat, rich in glutamine and glutamic acid. The involucrin gene,encoding a protein of the terminally differentiated keratinocyte, is polymorphic in the human. Involucrin has previously been mapped to chromosome 1q21. The epidermal protein involucrin has been remodeled in the higher primates. And the existence of two populations of East Asian involucrin alleles gives support for the existence of a Eurasian stem lineage from which Caucasians and a part of the Asian population originated.
Catalog #	I8447-27F
Applications	Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, and Immunofluorescence. Other applications not tested.
Recommended Dilution	Western Blot: 2ug/ml
	Immunohistochemistry(paraffin, frozen): 0.4-1ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	10K258
Host	Mouse
Source	Human
Concentration	As reported
Form	Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1.2% sodium acetate, PBS.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Human involucrin.
Specificity	Recognizes human IInvolucrin. Species Crossreactivity: porcine, canine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.