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You are here:Home » Growth Factors, Cytokines » Growth Factors-IP-10 » IP-10, Recombinant, Mouse (Gamma Interferon Inducible Protein-10)

IP-10, Recombinant, Mouse (Gamma Interferon Inducible Protein-10)

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Specifications

Among CXC chemokines, CXCL10 has been identified to play an important role in several endocrinological autoimmune diseases such as Hashimoto's thyroiditis, Graves'disease and Type 1 diabetes mellitus.The chemokine IP-10 (interferon-inducible protein of 10 kD, CXCL10) is a chemoattractant for CXCR3+ T cells, binds to the G protein-coupled receptor CXCR3, which is found mainly on activated T cells and NK cells, and plays an important role in Th1-type inflammatory diseases. IP-10 also binds to glycosaminoglycans (GAGs), an interaction thought to be important for its sequestration on endothelial and other cells.
Catalog #I8449-12A
DescriptionRecombinant Mouse IP-10 produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of 8701Dalton. Recombinant Murine IP-10 is purified by proprietary chromatographic techniques.
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Ile-Pro-Leu-Ala-Arg.
Dimers and AggregatesLess than 1% as determined by silver stained SDS-PAGE gel analysis.
Biological ActivityMurine IP-10 is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract IL-2 activated T cells at 0.1-10 ng/ml.
Endotoxin 0.1ng/ug (IEU/ug) of Mouse IP-10.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.02 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IP-10 as a Reference Standard.
SolubilityIt is recommended to reconstitute the lyophilized Mouse IP-10 in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized IP-10 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution IP-10 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Molecular Weight8.7kD
SourceE. coli
Purity97% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
ConcentrationAs reported
FormSupplied as a lyophilized powder without additives.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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