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You are here:Home » Antibodies » Abs to Enzymes, Phosphatase » Anti -IPP-1 (Inhibitor of Protein Phosphatase)

Anti -IPP-1 (Inhibitor of Protein Phosphatase)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH FC
The inhibitor of protein phosphatase 1 (IPP-1, I-1) is an endogenous inhibitor of protein phophastase 1 (PP-1), a signal transducer in many cellular pathways. IPP-1 is a member of heat-stable PP1 inhibitory subunits, which includes DARPP-32, (a homologue of IPP-1) and IPP-2. First discovered in rabbit skeletal muscle, IPP-1 is expressed in various mammalian tissues. IPP-1 inhibition of PP-1 is controlled through phosphorylation at Thr35 by cAMP-dependent protein kinase and a tetra-peptide consensus motif in various PP-1 binding proteins (1). IPP-1 is a hormonal control in muscle contraction, pituitary cell growth, synaptic plasticity by neurotransmitters, and glycogen metabolism (2). In vivo, IPP-1 is phosphorylated at Ser-67 by NCLK, which also initiates inhibition of PP-1 by IPP-1 (3). Triggered by increase in calcium level, IPP-1 is inactivated by PP2B through de-phosphorylation (4).
Catalog #I8465-02
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:80
Western Blot: 1:100,000
Immunohistochemistry: 1:100-250
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k231
HostRabbit
SourceHuman
FormSupplied as a liquid.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues in the N-terminus of human IPP-1
SpecificityRecognizes human IPP-1
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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