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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IP

Inositol 1,4,5-triphosphate receptor, also known as IP3R or InsP3R, is a member of the intracellular calcium release channel family, and is located in the endo- plasmic reticulum. It functions as a Ca2+ release channel for intracellular stores of calcium ions. There are three types of IP3 receptors (IP3 receptor 1, 2 and 3) that require the second messenger inositol 1,4,5-triphosphate (IP3) for activation (1). Four individual receptor subunits, resulting in homo- or hetero-oligomerization of the receptor isoform, form a functional channel (2). Phosphorylation of IP3R1 at Ser1756 by cyclic-AMP-dependent protein kinase A (PKA) regulates the sensitivity of IP3R1 to IP3 and may thus be a mode of regulation for Ca2+ release (3,4). IP3R1 mediated calcium release appears to have an effect on the induction of long term depression (LTD) in Purkinje cells (5).
Catalog #	I8471-01
Applications	Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000
	Immunoprecipitation: 1:50
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	As reported
Form	Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Synthetic peptide surrounding Met2750 of human IP3 receptor.
Specificity	Recognizes endogenous levels of total human IP3 receptor type 1 protein at ~320kD. SpecIes Crossreactivity: mouse, rat
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.