Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -IRS-1 (Insulin Receptor Substrate 1)

Anti -IRS-1 (Insulin Receptor Substrate 1)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP IH IC
Insulin Receptor Substrate (IRS-1) is one of the major substrates of Insulin Receptor Kinase. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2 domain-containing proteins (Grb2, p85, SHP-2, Fyn and others) and which mediate the metabolic and growth promoting functions of insulin. IRS-1 also contains over 30 potential Ser/Thr phosphorylation sites for kinases, such as PKA, PKC and MAPK. The phosphorylation of Ser 612 and Ser636/639 are mediated by PKA and mTOR pathways, respectively, resulting in an inhibition of insulin signaling in the cell and suggesting a potential mechanism for insulin resistance in some models of obesity. Once phosphorylated by insulin receptor, IRS-1 recruits the downstream components that mediate the insulin signaling. IRS-1 protein contains a conserved pleckstrin homology (PH) domain located at its amino terminus, adjacent to a phospho-tyrosine binding (PTB) domain.
Catalog #I8700-01C
ApplicationsSuitable for use in Immunoprecipitation, Immunocytochemistry and
Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunoprecipitation: 2-4ug immunoprecipitated IRS1 from 500ug of 3T3/A31 RIPA lysate.
Immunocytochemistry: 8ug/ml stained IRS1 in mouse brain cells fixed with 10% formalin as reported by an independent laboratory.
Immunohistochemistry: Reported by an independent laboratory to detect IRS1 in mouse brain sections.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No5H19
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, containing 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein G affinity chromatography.
ImmunogenFull length recombinant rat IRS-1 protein produced in baculovirus without tags (Swissprot ID# P35570).
SpecificityRecognizes rat IRS1, Mr 165kD. Species Crossreactivity: Mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links