| Insulin Receptor Substrate (IRS-1) is one of the major substrates of Insulin Receptor Kinase. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2 domain-containing proteins (Grb2, p85, SHP-2, Fyn and others) and which mediate the metabolic and growth promoting functions of insulin. IRS-1 also contains over 30 potential Ser/Thr phosphorylation sites for kinases, such as PKA, PKC and MAPK. The phosphorylation of Ser 612 and Ser636/639 are mediated by PKA and mTOR pathways, respectively, resulting in an inhibition of insulin signaling in the cell and suggesting a potential mechanism for insulin resistance in some models of obesity. Once phosphorylated by insulin receptor, IRS-1 recruits the downstream components that mediate the insulin signaling. IRS-1 protein contains a conserved pleckstrin homology (PH) domain located at its amino terminus, adjacent to a phospho-tyrosine binding (PTB) domain. |
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| Catalog # | I8700-01C |
| Applications | Suitable for use in Immunoprecipitation, Immunocytochemistry and |
| Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Immunoprecipitation: 2-4ug immunoprecipitated IRS1 from 500ug of 3T3/A31 RIPA lysate. |
| Immunocytochemistry: 8ug/ml stained IRS1 in mouse brain cells fixed with 10% formalin as reported by an independent laboratory. |
| Immunohistochemistry: Reported by an independent laboratory to detect IRS1 in mouse brain sections. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1,k |
| Clone No | 5H19 |
| Host | Mouse |
| Source | Rat |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, containing 0.05% sodium azide before the addition of glycerol to 30%. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Full length recombinant rat IRS-1 protein produced in baculovirus without tags (Swissprot ID# P35570). |
| Specificity | Recognizes rat IRS1, Mr 165kD. Species Crossreactivity: Mouse |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
