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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -IRS-1 (Insulin Receptor Substrate 1)

Anti -IRS-1 (Insulin Receptor Substrate 1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IC
Insulin Receptor Substrate 1 (IRS-1) is a cytoplasmic protein that participates in the insulin signaling pathway. It is phosphorylated by the Insulin receptor upon binding of Insulin and subsequently associates with several SH2-domain-containing proteins.
Catalog #I8700-01D
ApplicationsSuitable for use in Immunocytochemistry, Western Blot and ELISA. Other applications not tested.
Recommended DilutionImmunocytochemistry: 10ug/ml
Western Blot: 1-2ug/ml
ELISA: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No7H204
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile ddH2O or sterile 40-50% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenE. coli-derived recombinant human IRS-1 (aa801-1020) Accession # P35568
SpecificityRecognizes human IRS-1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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