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You are here:Home » Antibodies » Antibodies-Protein Kinase Substrates » Anti -IRS-1, phosphorylated (Tyr612) (Insulin Receptor Substrate 1)

Anti -IRS-1, phosphorylated (Tyr612) (Insulin Receptor Substrate 1)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Insulin Receptor Substrate-1 (IRS-1) is a 165kD multi-phosphorylated cytoplasmic docking protein involved in the regulation of metabolism and proliferation by insulin, IL-4 and other cytokines. Insulin binding to the insulin receptor activates the catalytic domain and causes phosphorylation on tyrosine residues of IRS proteins, which in turn serves to recruit a variety of Src homology-2 (SH2) domain-containing proteins such as PI3 kinase, Grb-2, SHP-2, Nck and Crk. These proteins further propagate intracellular signaling, culminating in both metabolic and growth-promoting functions of IRS-1. pTyr612 of IRS-1 is in a pYMxM consensus motif PI3 kinase binding site.
Catalog #I8700-02B
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Positive ControlInsulin treated human embryonic kidney cells or Chinese Hamster Ovary cells expressing human insulin receptor (293T or CHO-T, respectively) and transiently transfected with a plasmid encoding human IRS-1.
Storage and StabilityFor long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide.. 50% glycerol.
PurityPurified by affinity chromatography.
ImmunogenSynthetic phosphopeptide derived from a region of IRS-1 that contains tyrosine 612.
SpecificityRecognizes human IRS-1 when phosphorylated at Tyr612. Species sequence homology: Mouse, rat, and chicken 100%
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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