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Anti -IRS-1, phosphorylated (Ser307) (Insulin Receptor Substrate 1)
Anti -IRS-1, phosphorylated (Ser307) (Insulin Receptor Substrate 1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
B |
|
| IRS-1 is one of the major substrates of insulin receptor kinase. IRS-1 protein contains a conserved pleckstrin homology (PH) domain located at its amino terminus, adjacent to a phospho-tyrosine binding (PTB) domain. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2 domain-containing proteins (Grb2, p85, SHP-2, Fyn and others) and which mediate the metabolic and growth-promoting functions of insulin. IRS-1 also contains over 30 potential Ser/Thr phosphorylation sites for kinases, such as PKA, PKC and MAPK. Once phosphorylated by insulin receptor, IRS-1 recruits the downstream components that mediate the insulin signaling. | | | Catalog # | I8700-04 | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000-1:2000 detects phospho-IRS-1 (Ser307) in RIPA lysates from serum starved HEK 293 cells treated with 5ug/ml anisomycin for 30 minutes, but not in untreated cells. | | Optimal dilutions to be determined by the researcher. | | Immunoblot Analysis | HEK 293 cell lysates untreated and anisomycin stimulated were resolved by electrophoresis, transferred to nitrocellulose and probed with antiphospho-IRS-1 (Ser307) (1:1000 dilution) or anti-IRS-1 (1:1000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Rat | | Concentration | Not determined | | Form | Supplied as a liquid in TBS, 0.05% sodium azide before the addition of glycerol to 30%. | | Purity | Serum. | | Immunogen | Synthetic peptide corresponding to aa301-315 (ESITATpSPASMVGGK) of phospho-IRS-1 (Ser307) (KLH coupled). | | Specificity | Recognizes phospho-IRS-1 (Ser307), Mr 170kD. Species Crossreactivity | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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