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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -IRS-1, phosphorylated (Ser307) (Insulin Receptor Substrate 1)

Anti -IRS-1, phosphorylated (Ser307) (Insulin Receptor Substrate 1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain-containing proteins, which mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6). The phosphorylation of Ser612 and Ser636/639 are mediated by the PKC and mTOR pathways, respectively (7,8), resulting in an inhibition of insulin signaling in the cell and suggesting a potential mechanism for insulin resistance in some models of obesity.
Catalog #I8700-04A
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:1,000
Immunoprecipitation 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
PurityPurified by Protein A and peptide affinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to residues surrounding Ser307 of mouse IRS-1 (KLH coupled). Equivalent to human Ser312.
SpecificityDetects overexpressed or endogeneous mouse IRS-1 only when phosphorylated at Ser307 (Mr 175kD). Does not crossreact with other related phospho-proteins.
Species CrossreactivityPredicted to crossreact with human based on sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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