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You are here:Home » Antibodies » Antibodies-Protein Kinase Substrates » Anti -IRS-1, phosphorylated (Ser307) (Insulin Receptor Substrate 1) (Glycerol Free)

Anti -IRS-1, phosphorylated (Ser307) (Insulin Receptor Substrate 1) (Glycerol Free)


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Clone Host Grade Applications
Polyclonal Rabbit Serum B
IRS-1 is one of the major substrates of insulin receptor kinase. IRS-1 protein contains a conserved pleckstrin homology (PH) domain located at its amino terminus, adjacent to a phospho-tyrosine binding (PTB) domain. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2 domain-containing proteins (Grb2, p85, SHP-2, Fyn and others) and which mediate the metabolic and growth-promoting functions of insulin. IRS-1 also contains over 30 potential Ser/Thr phosphorylation sites for kinases, such as PKA, PKC and MAPK. Once phosphorylated by insulin receptor, IRS-1 recruits the downstream components that mediate the insulin signaling.
Catalog #I8700-04C
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:2000 detects phospho-IRS-1 (Ser307) in RIPA lysates from serum starved HEK 293 cells treated with 5ug/ml anisomycin for 30 minutes, but not in untreated cells. Optimal dilutions to be determined by the researcher.
Immunoblot AnalysisHEK 293 cell lysates untreated and anisomycin stimulated were resolved by electrophoresis, transferred to nitrocellulose and probed with antiphospho-IRS-1 (Ser307) (1:1000 dilution) or anti-IRS-1 (1:1000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationNot determined
FormSupplied as a liquid in TBS, 0.05% sodium azide. Glycerol free.
ImmunogenSynthetic peptide corresponding to aa301-315 (ESITATpSPASMVGGK) of phospho-IRS-1 (Ser307) (KLH coupled).
SpecificityRecognizes phospho-IRS-1 (Ser307), Mr 170kD. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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