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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -IRS-1, phosphorylated (Tyr941) (Insulin Receptor Substrate-1)

Anti -IRS-1, phosphorylated (Tyr941) (Insulin Receptor Substrate-1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Untreated (Lane 1) or insulin treated serum starved (Lanes 2) 293 cell lysates were resolved by electrophoresis, transferred to PVDF, and probed with anti-phospho-IRS1 (Tyr941) for 2 hours at room temperature. Proteins were visualized via HRP and chemiluminescent detection. Arrow
indicates phospho-IRS1.
Catalog #I8700-05G
Molecular Weight 165kD
Applications:
Suitable for use in Western Blot.
Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
FormSupplied as a liquid PBS, pH 7.3 containing 1.0 mg/mL BSA, 0.05% sodium azide and 50% glycerol.
PurityPurified by immunoaffinity chromatography
ImmunogenSynthetic peptide corresponding to amino acid region encompassing the human (Tyr941), mouse (Tyr935), and rat phospho-IRS1.
SpecificityRecognizes phosphorylated IRS1 (Tyr941)
Species Cross-reactivityHuman
Species Sequence HomologyMouse, Rat (100% homologous)
and chicken (86% homologous)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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