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Anti -IRS-1, phosphorylated (Tyr941) (Insulin Receptor Substrate-1)
Anti -IRS-1, phosphorylated (Tyr941) (Insulin Receptor Substrate-1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| Untreated (Lane 1) or insulin treated serum starved (Lanes 2) 293 cell lysates were resolved by electrophoresis, transferred to PVDF, and probed with anti-phospho-IRS1 (Tyr941) for 2 hours at room temperature. Proteins were visualized via HRP and chemiluminescent detection. Arrow | | | indicates phospho-IRS1. | | | Catalog # | I8700-05G | | Molecular Weight | 165kD | | | | Applications: | | Suitable for use in Western Blot. | | Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 | | Optimal dilutions to be determined by researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Form | Supplied as a liquid PBS, pH 7.3 containing 1.0 mg/mL BSA, 0.05% sodium azide and 50% glycerol. | | | | Purity | Purified by immunoaffinity chromatography | | Immunogen | Synthetic peptide corresponding to amino acid region encompassing the human (Tyr941), mouse (Tyr935), and rat phospho-IRS1. | | Specificity | Recognizes phosphorylated IRS1 (Tyr941) | | Species Cross-reactivity | Human | | Species Sequence Homology | Mouse, Rat (100% homologous) | | and chicken (86% homologous) | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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