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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -IRS-1, phosphorylated (Tyr941) (Insulin Receptor Substrate-1)

Anti -IRS-1, phosphorylated (Tyr941) (Insulin Receptor Substrate-1)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Untreated (Lane 1) or insulin treated serum starved (Lanes 2) 293 cell lysates were resolved by electrophoresis, transferred to PVDF, and probed with anti-phospho-IRS1 (Tyr941) for 2 hours at room temperature. Proteins were visualized via HRP and chemiluminescent detection. Arrow
indicates phospho-IRS1.
Catalog #I8700-05G
Molecular Weight 165kD
Suitable for use in Western Blot.
Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid PBS, pH 7.3 containing 1.0 mg/mL BSA, 0.05% sodium azide and 50% glycerol.
PurityPurified by immunoaffinity chromatography
ImmunogenSynthetic peptide corresponding to amino acid region encompassing the human (Tyr941), mouse (Tyr935), and rat phospho-IRS1.
SpecificityRecognizes phosphorylated IRS1 (Tyr941)
Species Cross-reactivityHuman
Species Sequence HomologyMouse, Rat (100% homologous)
and chicken (86% homologous)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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