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Anti -IRS-1, phosphorylated-IRS1 (Tyr1179) (Insulin Receptor Substrate-1)
Anti -IRS-1, phosphorylated-IRS1 (Tyr1179) (Insulin Receptor Substrate-1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| Untreated (Lane 1) or insulin treated (Lanes 2 and 3) lysates from CHO cells transfected with IRS11 were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-phospho-IRS1 (Tyr1179) for 2 hours at room temperature. Lane 3 was pre- incubated with the blocking peptide immunogen corresponding to phospho-IRS1 (Tyr1179). Proteins | | | were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-IRS1. | | | Catalog # | I8700-05N | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | 1:1000. Optimal dilution to be determined by researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Form | Supplied in PBS, pH 7.3, containing 1mg/mL BSA, 0.05% sodium azide and 50% glycerol. | | Purity | Purified by immunoaffinity chromatograpy. | | Immunogen | Synthetic peptide corresponding to amino acid region encompassing the human and mouse phospho- IRS1 (Tyr1179), | | Specificity | Recognizes phosphorylated IRS1 (Tyr1179), Mr 165kD. | | Species Cross-reactivity | Human | | Species Sequence Homology | Mouse, Rat and chicken. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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