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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -IRS-1, phosphorylated-IRS1 (Tyr1179) (Insulin Receptor Substrate-1)

Anti -IRS-1, phosphorylated-IRS1 (Tyr1179) (Insulin Receptor Substrate-1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Untreated (Lane 1) or insulin treated (Lanes 2 and 3) lysates from CHO cells transfected with IRS11 were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-phospho-IRS1 (Tyr1179) for 2 hours at room temperature. Lane 3 was pre- incubated with the blocking peptide immunogen corresponding to phospho-IRS1 (Tyr1179). Proteins
were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-IRS1.
Catalog #I8700-05N
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended Dilution1:1000. Optimal dilution to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
FormSupplied in PBS, pH 7.3, containing 1mg/mL BSA, 0.05% sodium azide and 50% glycerol.
PurityPurified by immunoaffinity chromatograpy.
ImmunogenSynthetic peptide corresponding to amino acid region encompassing the human and mouse phospho- IRS1 (Tyr1179),
SpecificityRecognizes phosphorylated IRS1 (Tyr1179), Mr 165kD.
Species Cross-reactivityHuman
Species Sequence HomologyMouse, Rat and chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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