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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -IRS-1, phosphorylated (Tyr1229) (Insulin Receptor Substrate 1)

Anti -IRS-1, phosphorylated (Tyr1229) (Insulin Receptor Substrate 1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #I8700-05W
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Quality Control Testing Immunoblot Analysis 0.5 g/mL of this lot detected phospho-IRS1 (Tyr1229) in RIPA lysates from insulin treated IRS1 transfected CHO cells. Insulin treated lysates from CHO cells transfected with human IRS1 were resolved by electrophoresis, transferred to nitrocellulose, and probed with antiphospho- IRS1 (Tyr1229) for 2 hours at room temperature. Lane 2 blot was pre-incubated with phosphatase. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-IRS1.
Clone TypePolyclonal
HostRabbit
ConcentrationNot determined
FormSupplied as a liquid in PBSpH 7.4 containing 1.0 mg/mL BSA and 0.05% sodium azide).
PurityPurified by immunoaffinity chromatography.
ImmunogenPeptide corresponding to amino acid region encompassing the human phospho-IRS-1 (Tyr1229).
SpecificityRecognizes phosphorylated IRS-1(Tyr1229), Mr 165 kDa. Species Cross-reactivity: Human tested. Predicted to cross-react with Mouse and Rat based on sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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