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Anti -IRS-1, phosphorylated (Tyr1179) (Insulin Receptor Substrate 1)
Anti -IRS-1, phosphorylated (Tyr1179) (Insulin Receptor Substrate 1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| Catalog # | I8700-05X | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Quality Control Testing Immunoblot Analysis | A 1:1000 dilution of this lot detected phospho-IRS1 (Tyr1179) in RIPA lysates from insulin stimulated IRS1 transfected CHO cells. Untreated (Lane 1) or insulin treated (Lanes 2 and 3) lysates from CHO cells transfected with IRS11 were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-phospho-IRS1 (Tyr1179) for 2 hours at room temperature. Lane 3 was preincubated with the blocking peptide immunogen corresponding to phospho-IRS1 (Tyr1179). Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-IRS1. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Concentration | As reported | | Form | Supplied as a liquid in PBS (withoutMg2+ and Ca2+), pH 7.2 1mg/ml BSA(IgG, protease free), 0.05% sodium azide), 50% glycerol. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Peptide corresponding to amino acid region encompassing the human and mouse phospho- IRS1 (Tyr1179), | | Specificity | Recognizes phosphorylated IRS1(Tyr1179), Mr 165 kDa | | Species Cross-reactivity | Human tested. Predictedto cross-react with Mouse (100% homologous), Rat(93% homologous), and chicken (79% homologous) based on sequence homology. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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