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Anti -IRS-1, phosphorylated (Tyr1229) (Insulin Receptor Substrate-1)
Anti -IRS-1, phosphorylated (Tyr1229) (Insulin Receptor Substrate-1)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| Insulin treated lysates from CHO cells transfected with human IRS1 were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-phospho-IRS1 (Tyr1229) | | | for 2 hours at room temperature. Lane 2 blot was pre-incubated with phosphatase. Proteins | | | were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-IRS1. | | | Catalog # | I8700-05Y | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 0.5 g/mL of this lot detected phospho-IRS1 (Tyr1229) in RIPA lysates from insulin treated IRS1 transfected CHO cells. | | Optimal dilution to be determined by researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Form | Supplied in PBS, pH 7.4 containing 1mg/mL BSA and 0.05% sodium azide. | | Purity | Purified by immunoaffinity chromatography | | Immunogen | Synthetic peptide corresponding to amino acid region encompassing the human phospho-IRS-1 (Tyr1229). | | Specificity | Recognizes phosphorylated IRS-1 (Tyr1229), Mr 165kD. | | Species Cross-reactivity | Human | | Species Sequence Homology | Mouse and Rat | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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