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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -IRS-1, phosphorylated (Tyr1229) (Insulin Receptor Substrate-1)

Anti -IRS-1, phosphorylated (Tyr1229) (Insulin Receptor Substrate-1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Insulin treated lysates from CHO cells transfected with human IRS1 were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-phospho-IRS1 (Tyr1229)
for 2 hours at room temperature. Lane 2 blot was pre-incubated with phosphatase. Proteins
were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-IRS1.
Catalog #I8700-05Y
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5 g/mL of this lot detected phospho-IRS1 (Tyr1229) in RIPA lysates from insulin treated IRS1 transfected CHO cells.
Optimal dilution to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
FormSupplied in PBS, pH 7.4 containing 1mg/mL BSA and 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography
ImmunogenSynthetic peptide corresponding to amino acid region encompassing the human phospho-IRS-1 (Tyr1229).
SpecificityRecognizes phosphorylated IRS-1 (Tyr1229), Mr 165kD.
Species Cross-reactivityHuman
Species Sequence HomologyMouse and Rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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