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You are here:Home » Antibodies » Antibodies-Protein Kinase Substrates » Anti -IRS-1 (Insulin Receptor Substrate 1) (Agarose)

Anti -IRS-1 (Insulin Receptor Substrate 1) (Agarose)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified IP
Insulin Receptor Substrate (IRS-1) is one of the major substrates of Insulin Receptor Kinase. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2 domain-containing proteins (Grb2, p85, SHP-2, Fyn and others) and which mediate the metabolic and growth promoting functions of insulin. IRS-1 also contains over 30 potential Ser/Thr phosphorylation sites for kinases, such as PKA, PKC and MAPK. The phosphorylation of Ser 612 and Ser636/639 are mediated by PKA and mTOR pathways, respectively, resulting in an inhibition of insulin signaling in the cell and suggesting a potential mechanism for insulin resistance in some models of obesity. Once phosphorylated by insulin receptor, IRS-1 recruits the downstream components that mediate the insulin signaling. IRS-1 protein contains a conserved pleckstrin homology (PH) domain located at its amino terminus, adjacent to a phospho-tyrosine binding (PTB) domain.
Catalog #I8700-06
Immunoprecipitation 50ul immunoprecipitates IRS-1 from 500ug of a RIPA lysate of 3T3/A31 mouse fibroblasts. After boiling in SDS-PAGE sample buffer a small amount of the non-covalently bound IgG light chain (28kDa) and heavy chain (55kDa) may be observed.
Elution Buffer % IRS-1 Released SDS-Page sample buffer 100 0.5M Glycine, pH 2, 0 0.1M TEA, pH 11.5.
Affinity Purification The antibody-agarose may be useful for affinity purification of proteins that bind IRS-1.
Clone TypePolyclonal
FormProvided as a 50% gel slurry in PBS, 0.05% azide.
PurityPurified by affinity chromatography.
ImmunogenCarboxy-terminal 14 amino acid peptide of rat liver IRS-1 (CYASINFQKQPEDRQ).
SpecificityRecognizes rat IRS-1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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