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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -IRS1, phosphorylated (Ser639) (Insulin Receptor Substrate 1, IRS-1)

Anti -IRS1, phosphorylated (Ser639) (Insulin Receptor Substrate 1, IRS-1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified IH
IRS-1 regulates most insulin responses, including peripheral and central glucose homeostasis, protein synthesis, and cell proliferation and growth. The protein contains an N-terminal pleckstrin homology (PH) domain and phosphotyrosine-binding (PTB) domain which are necessary for targeting IRS-1 to the activated insulin receptor at plasma membrane, where it becomes phosphorylated in response to insulin stimulation of cells. Once phosphorlated by the insulin receptor, the protein binds specifically to various cellular proteins containing Src homology-2 (Sh2) domain, including phosphatidylinositol 3-kinase (PI-3K), Grb2-Sos, and protein tyrosine phosphatase type 11 (PTPN11 or SHP2). In humans, the pathological role of the protein has been detected in non-insulin-dependent diabetes mellitus.
Catalog #I8700-06E
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin fixed paraffin embedded): 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to human IRS-1 around the phosphorylation site of serine 639 (P-K-SP-V-S).
SpecificityRecognizes human IRS-1, phosphorylated at Ser639. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesHIRS-1


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