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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -IRS-1 (Insulin Receptor Substrate 1)

Anti -IRS-1 (Insulin Receptor Substrate 1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
IRS-1 (Insulin receptor substrate-1) is phosphorylated in response to stimulation of cells by insulin. Upon ligand-induced phosphorylation, IRS-1 immediately associates with a series of SH2 domain-containing signaling intermediates such as PI3-kinase, GRB2, Syp and Nck. The level of diversity in structure and function of the SH2-containing proteins which binds activated IRS-1 is important for responsiveness to insulin. IRS phosphorylation elicits the growth and metablic effects of insulin growth factors and insulin by allowing glucose uptake, glycogen synthesis and mitogenic activity. IRS-1 migrates between 170 and 185kD and is expressed in most cell types.
Catalog #I8700-07A
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1-2 ug/ml
Optimal dilutions to be determined by the researcher.
Control Peptide I8700-07A1
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 1% BSA, 0.02% thimerosal, 50% glycerol.
PurityPurified by peptide affinitiy chromatography.
ImmunogenSynthetic peptide mapping to the C-terminus of human IRS-1
SpecificityRecognizes human IRS-1 at ~175kD. It does not cross-react with IRS-2, -3, and -4. Species crossreactivity: mouse, rat, monkey, horse, dog, pig
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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