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You are here:Home » Antibodies » Antibodies-Protein Kinase Substrates » Anti -IRS-2, phosphorylated (Ser731) (Insulin Receptor Substrate 2) (Azide and BSA Free)

Anti -IRS-2, phosphorylated (Ser731) (Insulin Receptor Substrate 2) (Azide and BSA Free)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP
Insulin exerts its effects by binding to the insulin receptor, a disulfide-linked heterotetrameric protein comprised of two alpha subunits and two beta subunits arranged in the following configuration: beta-alpha-alpha-beta. The alpha subunits each contain insulin binding sites and are entirely extracellular in localization. The beta subunits each possess an extracellular domain, a single transmembrane domain, and a cytoplasmic tyrosine kinase domain. Binding of insulin to the alpha subunits induces a conformation change in the receptor which activates the kinase domain, stimulating tyrosine autophosphorylation of the receptor and tyrosine phosphorylation of at least five different insulin receptor substrates designated IRS-1-4, and Shc. This tyrosine phosphorylation produces docking sites for proteins bearing SH2 domains, such as PI3-K, Grb-2, Nck, and Crk. While tyrosine phosphorylation of insulin receptor substrates propagates signaling from insulin, serine and threonine phosphorylation by MAPK’s, PKA, and PKC may reduce insulin signaling.
Catalog #I8700-18A
ApplicationsSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionELISA: 0.1-1.0ug/ml
Immunoprecipation: 3-5ug/extract from 10e7 cells
Western Blot: 0.5-2.0ug/ml
Optimal dilutions to be determined by the researcher.
Recommended Positive ControlSerum-treated mouse fibroblast (3T3) cells.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.2. No preservative added. Also available with azide. See I8700-18.
PurityPurified from rabbit serum by epitope affinity chromatography.
ImmunogenSynthetic phosphopeptide derived from the human IRS-2 sequence, corresponding to the serine 731 phosphorylation site. This site corresponds to serine 133 in mouse and serine 233 in rat.
SpecificityRecognizes human IRS-2 when phosphorylated at serine 731.
Species CrossreactivityMouse, and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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