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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -IRS-4 (Insulin Receptor Substrate 4)

Anti -IRS-4 (Insulin Receptor Substrate 4)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
Insulin Receptor Substrate 4 (IRS-4) is a new member of the insulin receptor substrate family. IRS-4 is a 133kD protein that undergoes rapid tyrosine phosphorylation in response to insulin. IRS-4 was originally discovered and characterized in human embryonic kidney (HEK) 293 cells where it is the predominant insulin-dependent phosphotyrosine protein. Subcellular fractionation of HEK 293 cells revealed that about 50% of IRS-4 is located in cellular membranes whereas immunofluoresence showed that IRS-4 is concentrated at the plasma membrane.
Catalog #I8700-25
ApplicationsSuitable for use in Immunoblot and Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunoblot Analysis: 0.5-2.0ug/ml detected IRS-4 in RIPA lysates from human embryonic kidney (HEK) cells and rat L6 cells. The IRS-4 protein band appears to run at a slightly lower MWapp on minigels than is reported in literature. HEK cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-rabbit IRS-4 (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation 4ug immunoprecipitated IRS-4 from 500ug of human embryonic kidney RIPA lysate.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenKLH conjugated synthetic peptide (CRMDFARRDNQFDSPKRGR) corresponding to amino acids 1240-1257 of human IRS-4. The immunizing sequence does not share any significant homology with IRS-1, IRS-2 or IRS-3.
SpecificityRecognizes IRS-4, Mr 133kD-160kD, due to multiple phosphorylation sites. Specificity for IRS-4 was demonstrated by using the immunizing peptide to inhibit antibody binding. Species Crossreactivity: Human and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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