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Anti -IRS-4 (Insulin Receptor Substrate 4)
Anti -IRS-4 (Insulin Receptor Substrate 4)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IP |
|
| Insulin Receptor Substrate 4 (IRS-4) is a new member of the insulin receptor substrate family. IRS-4 is a 133kD protein that undergoes rapid tyrosine phosphorylation in response to insulin. IRS-4 was originally discovered and characterized in human embryonic kidney (HEK) 293 cells where it is the predominant insulin-dependent phosphotyrosine protein. Subcellular fractionation of HEK 293 cells revealed that about 50% of IRS-4 is located in cellular membranes whereas immunofluoresence showed that IRS-4 is concentrated at the plasma membrane. | | | Catalog # | I8700-25 | | Applications | Suitable for use in Immunoblot and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Immunoblot Analysis: 0.5-2.0ug/ml detected IRS-4 in RIPA lysates from human embryonic kidney (HEK) cells and rat L6 cells. The IRS-4 protein band appears to run at a slightly lower MWapp on minigels than is reported in literature. HEK cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-rabbit IRS-4 (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. | | Immunoprecipitation | 4ug immunoprecipitated IRS-4 from 500ug of human embryonic kidney RIPA lysate. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | KLH conjugated synthetic peptide (CRMDFARRDNQFDSPKRGR) corresponding to amino acids 1240-1257 of human IRS-4. The immunizing sequence does not share any significant homology with IRS-1, IRS-2 or IRS-3. | | Specificity | Recognizes IRS-4, Mr 133kD-160kD, due to multiple phosphorylation sites. Specificity for IRS-4 was demonstrated by using the immunizing peptide to inhibit antibody binding. Species Crossreactivity: Human and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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