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You are here:Home » Cloning » Cloning-Enzymes » Isocitrate Dehydrogenase,Taq, Thermostable, Recombinant (ICDH-Taq)

Isocitrate Dehydrogenase,Taq, Thermostable, Recombinant (ICDH-Taq)

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Specifications

Isocitrate Dehydrogenase is an enzyme of the oxidoreductase class that catalyzes the conversion of isocitrate and NAD+ to yield 2-ketoglutarate, carbon dioxide, and NADH. It occurs in cell mitochondria. The enzyme requires Mg2+, Mn2+; it is activated by ADP, citrate, and Ca2+, and inhibited by NADH, NADPH, and ATP. The reaction is the key rate-limiting step of the citric acid (tricarboxylic) cycle. Recombinant MDH is purified by proprietary chromatographic techniques.
Catalog #I8850-01B
ApplicationSuitable for use as a calibrator or control serum.
Dimers and Aggregates 1% as determined by silver-stained SDS-PAGE gel analysis. Endotoxin: 0.1ng/ug (IEU/ug) of riCDH.
Specific Activity 20u/mg protein (25°C, pH 8.5)
Unit DefinitionOne unit is defined as 1 mol of NAD+ production per minute under the assay conditions (25°C, pH 7.5).
Standard rICDH(Taq)-03
Contaminants (as rICDH activity = 100%)
NADPH oxidase 0.01%
Phosphatase 0.00015%
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
SourceE. coli
PurityHighly Purified
FormLyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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