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Isocitrate Dehydrogenase,Taq, Thermostable, Recombinant (ICDH-Taq)
Isocitrate Dehydrogenase,Taq, Thermostable, Recombinant (ICDH-Taq)
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| Isocitrate Dehydrogenase is an enzyme of the oxidoreductase class that catalyzes the conversion of isocitrate and NAD+ to yield 2-ketoglutarate, carbon dioxide, and NADH. It occurs in cell mitochondria. The enzyme requires Mg2+, Mn2+; it is activated by ADP, citrate, and Ca2+, and inhibited by NADH, NADPH, and ATP. The reaction is the key rate-limiting step of the citric acid (tricarboxylic) cycle. Recombinant MDH is purified by proprietary chromatographic techniques. | | | Catalog # | I8850-01B | | Application | Suitable for use as a calibrator or control serum. | | Dimers and Aggregates | 1% as determined by silver-stained SDS-PAGE gel analysis. Endotoxin: 0.1ng/ug (IEU/ug) of riCDH. | | Specific Activity | 20u/mg protein (25°C, pH 8.5) | | Unit Definition | One unit is defined as 1 mol of NAD+ production per minute under the assay conditions (25°C, pH 7.5). | | Standard | rICDH(Taq)-03 | | Contaminants | (as rICDH activity = 100%) | | NADPH oxidase 0.01% | | Phosphatase 0.00015% | | Storage and Stability | Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months. | | Source | E. coli | | Purity | Highly Purified | | Form | Lyophilized powder. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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