| Isoprostanes are prostaglandin-like compounds that are produced by free radical mediated peroxidation of lipoproteins. This kit is for the quantification of a representative isoprostane, 15-isoprostane F2t (also known as 8-epi-PGF2 or 8-iso-PGF2) Levels of 15-isoprostane F2t in biological fluids have been shown to be useful for assessment of oxidant stress in vivo. 15- isoprostane F2t has also been shown to be a potent vasoconstrictor in rat kidneys and rabbit lungs, and plays a causative role in atherogenesis. Elevated isoprostane levels are associated with hepatorenal syndrome, rheumatoid arthritis, atherosclerosis, and carcinogenesis. |
|
| Catalog # | I9050 |
| This kit can be used for the quantification of free 15-isoprostane F2t in urine, serum or tissue samples following solid phase extraction of the isoprostane-containing fraction. Instructions are also provided for the quantification of total 15-isoprostane F2t following hydrolysis of phospholipids. |
| Principle | This kit is a competitive ELISA for determining levels of 15-isoprostane F2t in biological samples. Briefly, 15-isoprostane F2t in the samples or standards competes with 15-isoprostane F2t conjugated to HRP (HRP) for binding to a polyclonal antibody specific for 15-isoprostane F2t coated on the microplate. The HRP activity results in color development when substrate is added, with the intensity of the color proportional to the amount of 15-isoprostane F2t bound and inversely proportional to the amount of unconjugated 15-isoprostane F2t the samples or standards.The sensitivity of the assay is 0.1ng/ml, and the dynamic range is 0.1-1.0ng/ml. |
| Kit Components | I9050A: Microtiter Plate 1x96wells |
| I9050B: 15-isoprostane F2t Standard 2x60ul |
| I9050C: Wash Buffer (5X) 1x40ml |
| I9050D: Dilution Buffer (5X) 1x100ml |
| I9050E: Substrate 1x25ml |
| I9050F: 15-isoprostane F2t (HRP)1x100ul |
| Storage and Stability | Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| |
| Sensitivity | 0.1ng/ml |
|
| Dynamic Range | 0.1-1.0ng/ml |
|
| Performance Characteristics | |
| Cross reactivity at 50% B/B0 | |
| 15-isoprostane F2t 100.0% |
| 9 ,11-Prostaglandin F2 4.1% |
| 13,14-Dihydro-15-KETO-PGF2 3.0% |
| 9 ,11-Prostaglandin F2 <0.01% |
| Prostaglandin F2 <0.01 |
| 6-KETO-Prostaglandin F1 <0.01% |
| Prostaglandin E2 <0.01% |
| Prostaglandin D2 <0.01% |
| Arachidonic Acid <0.01% |
|
|
