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Isoprostane (8-iso-PGF2a) Urine ELISA Kit, BioAssay™
Isoprostane (8-iso-PGF2a) Urine ELISA Kit, BioAssay™
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| Isoprostanes are prostaglandin-like compounds that are produced by free radical mediated peroxidation of lipoproteins. This kit is for the quantification of 15-isoprostane F2t (also known as 8-epi-PGF2a or 8-iso-PGF2a) in urine samples. Levels of 15-isoprostane F2t in urine are useful for the non-invasive assessment of oxidant stress in vivo. 15-isoprostane F2t has also been shown to be a potent vasoconstrictor in rat kidneys and rabbit lungs, and plays a causative role in atherogenesis. Elevated isoprostane levels are associated with hepatorenal syndrome, rheumatoid arthritis, atherosclerosis, and carcinogenesis. This kit can be used for the quantification of free 15-isoprostane F2t in urine serum samples without the need for prior purification or extraction. | | | Catalog # | I9055 | | Principle | This kit is a competitive ELISA for determining levels of 15-isoprostane F2t (the best characterized isoprostane) in urine samples. Briefly, urine samples are mixed with an enhancing reagent that essentially eliminates interferences due to non-specific binding, and the 15-isoprostane F2t in the samples or standards competes with 15-isoprostane F2t conjugated to HRP (HRP) for binding to a polyclonal antibody specific for 15-isoprostane F2t coated on the microplate. The HRP activity results in color development when substrate is added, with the intensity of the color proportional to the amount of 15-isoprostane F2t bound and inversely proportional to the amount of unconjugated 15-isoprostane F2t in the samples or standards. | | Kit Components | | | I9055-01: Microtiter plate precoated with 15-isoprostane F2t 1x96wells | | I9055-02: 15-isoprostane F2t Standard 2 x 60ul | | I9055-03: Enhancer Reagent 1x100ml | | I9055-04: Wash Buffer (5X) 1x40ml | | I9055-05: TMB Substrate 1x25ml | | I9055-06: 15-isoprostane F2t (HRP) 1x250ul | | Storage and Stability | Store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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