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You are here:Home » Antibodies » Abs to Notch Proteins » Anti -Jagged 1 (JAG1, AGS, AHD, AWS, HJ1, JAGL1, Alagille Syndrome Protein, CD339, Headturner, Htu, MGC104644, Serrate-1, Ser-1, Slalom)

Anti -Jagged 1 (JAG1, AGS, AHD, AWS, HJ1, JAGL1, Alagille Syndrome Protein, CD339,
Headturner, Htu, MGC104644, Serrate-1, Ser-1, Slalom)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
Jagged 1 is a 180kD type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218aa precursor that contains a 33aa signal sequence, a 1034aa extracellular domain (ECD), a 26aa transmembrane segment, and a 125aa cytoplasmic region. The ECD contains a DSL domain (aa185-229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 shares 97-96aa identity with mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature. Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance.
Catalog #J0400-01K
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 2.5ug labels 10e6 cells. Using K562 human chronic myelogenous leukemia cell line, bEnd.3 mouse endothelioma cell line and rat cortical stem cells.
Optimal dilutions to be determined by the researcher.
HybridomaNSO myeloma cells with B cells from Balb/c mice.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C after receipt. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No11n115
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 1ml sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa32-296 from human Jagged 1 expressed in NSO cells (P78504).
SpecificityRecognizes human Jagged 1. Does not recognize recombinant human Jagged 2. Stains human Jagged 1 transfectants but not the parental cell line or human Jagged 2 transfectants in Flow Cytometry. Species Crossreactivity: mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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