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You are here:Home » Antibodies » Abs to Tyrosine Kinases » Anti -JAK2 (Janus Kinase 2)

Anti -JAK2 (Janus Kinase 2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IP
The Janus family of tyrosine kinases (JAK) includes JAK1, JAK2, JAK3 and Tyk2. This family associates with a variety of cytokine receptors and is activated by ligand binding. Upon ligand binding to cytokine receptors, JAKs phosphorylate themselves and their associated receptors. This provides multiple binding sites for signaling proteins containing SH2 or other phospho-tyrosine-binding domains, including STATs, Shc, insulin receptor substrates and FAK.
Catalog #J0901-04
ApplicationsSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000; Detects JAK2 in a modi ed RIPA lysate of rat L6 myoblasts. Detected JAK2 in modi ed RIPA lysates of mouse 3T3/A31 broblasts and human A431 cells. L6 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probe with anti-JAK2. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: 5ul immunoprecipitates JAK2 from 500ug of a rat L6 cellular lysate prepared in a modi ed RIPA buffer.
Optimal dilutions to be determined by the researcher.
ControlsHeLa, Jurkat, RIPA or A431 cell lysates
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationNot determined
FormSupplied as a liquid in buffer with 0.01% sodium azide.
PuritySerum
ImmunogenSynthetic peptide, aa758-776 of murine JAK2.
SpecificityRecognizes mouse JAK2 (130kD). Additional lower MW bands may be detected in some lysates. Species Crossreactivity: Human and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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