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You are here:Home » Antibodies » Abs to Tyrosine Kinases » Anti -JAK2, phosphorylated (Janus Kinase 2) (Y1007, Y1008)

Anti -JAK2, phosphorylated (Janus Kinase 2) (Y1007, Y1008)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
The Janus (JAK) family of cytoplasmic tyrosine kinases has been implicated in signal transduction induced by cytokines and growth factors, physically associated with ligand-bound receptors. This association that results in tyrosine phosphorylation and activation. JAK1 is approximately 130kD and contains a C-terminal tyrosine kinase domain, an adjacent kinase or kinase-related domain, and five other domains that are highly conserved among JAK family members. Family members such as JAK1, JAK2, and TYK2 are ubiquitous, whereas JAK3 is predominantly expressed in T lymphocytes. Studies with mutant cells that do not express JAK1, JAK2, or TYK2 show that their activation is essential for cellular signaling. JAK family kinases may either phosphorylate each other or be phosphorylated by a yet undescribed tyrosine kinase. Different cytokines can activate via phosphorylation distinct JAK family members. In some cells, the membrane-associated gp130 protein has been implicated in the induction of JAK family phosphorylation, although gp130 itself has no known activity.
Catalog #J0901-10A
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-2.0ug/ml. Detects phospho JAK2 in RIPA lysates from HEK293 cells expressing co-transfected JAK2 and chimeric Epo-Leptin receptors, stimulated with Epo. Phospho JAK 2 ~130kD.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to amino acids surrounding phosphotyrosines 1007 and 1008 of human JAK2 (KLH coupled).
SpecificityRecognizes phosphorylated JAK2, Mr 130kD. Species Crossreactivity: Human and mouse. Predicted to crossreact with rat based on sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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