| Janus Kinase 2 (JAK2) is a 130kD tyrosine kinase involved in cytoplasmic signal transduction. |
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| Ligand binding to a variety of cell surface receptors (e.g., cytokine, growth factor, GPCRs) leads to |
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| an association of those receptors with JAK proteins, which are then activated via phosphorylation on tyrosines 1007 and 1008 in the kinase activation loop. Activated JAK proteins phosphorylate and activate STAT (signal transducers and activators of transcription) proteins, which then dimerize and translocate to the nucleus. Once in the nucleus, STAT proteins bind to DNA and modify the transcription of various genes, which can lead to various responses such as cell proliferation, cell survival and differentiation. |
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| Catalog # | J0901-10B |
| Applications | Suitable for use in Western Blot.. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:1000 |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | 3T3-L1 adipocytes +/- Leukemia Inhibitory Factor (LIF). |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Host | Rabbit |
| Source | Human |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.3, 0.05% sodium azide, 50% glycerol. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic phosphopeptide derived from a region of JAK2 that contains tyrosines 1007 and 1008. |
| Specificity | Recognizes JAK2, phosphorylated (Tyr1007, Tyr1008) (Janus Kinase 2). Species Crossreactivity: human, mouse and rat JAK2. Other species have not been tested. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
