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You are here:Home » Antibodies » Abs to Enzymes, Methyltransferase » Anti -JBP1 Methyltransferase (Skb1Hs)

Anti -JBP1 Methyltransferase (Skb1Hs)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
Human Skb1Hs methyltransferase (also called JBP1), a homologue of yeast protein Skb1 and Hsl7p (1,2), is composed of 637 amino acid residues and contains motifs conserved among protein methyltransferases. It methylates histones and MBP in vitro (2). Yeast Hsl7p is involved in regulation of cell cycle progression through G2 by negatively regulating Swe1p, a protein tyrosine kinase that phosphorylates and inhibits Cdc28p (3). An Hsl7p homologue, Skb1, was identified in fission yeast by virtue of its yeast twohybrid interaction with Shk1p, a p21 (cdc42p/Rac) activated kinase (PAK) (4). Both proteins belong to the protein methyltransferase superfamily (5). Interestingly, human Skb1Hs methyltransferase was shown to interact with Jak kinases. This suggests the possibility that the Skb1Hs methyltransferase could link Jak to a PAK signaling pathway in mammalian cells.
Catalog #J1100
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:1000
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined.
FormSupplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA and 50% glycerol. No preservative added.
PurityPurified by Protein A and peptide affinity chromatography.
ImmunogenSynthetic peptide corresponding to residues surrounding aa100 of human JBP1 Methyltransferase (KLH). MW: 70kD.
SpecificityRecognizes endogenous levels of total human Skb1Hs methyltransferase protein at 70kD. Does not crossreact with other related mammalian methyltransferases. Species Crossreactivity: monkey, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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