| J Chain serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also help to bind these immunoglobulins to secretory component. |
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| Catalog # | J1255 |
| Applications | Suitable for use in Immunohistochemistry, ELISA, Immunoprecipitation and Western Blot. Other applications have not been tested. |
| Recommended Dilution | Immunohistochemistry (Frozen): 1:200 |
| ELISA: 20ug/ml |
| Immunoprecipitation: 20ug/ml |
| Optimal working dilutions to be determined by researcher. |
| Hybridoma | NS1 myeloma cells with spleen cells from Balb/c mice. |
| Recommended Secondary Antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-14C: IgG, Fc (HRP) Pab Gt xMo |
| I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H2560 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.0, 0.09% sodium azide. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Human J Chain. |
| Specificity | Recognizes human J chain as tested by ELISA using purified human Ig fragments as the solid phase target antigen. The antibody does not detect J chain in intact IgM in ELISA assays due to steric hindrance. In Western Blot techniques the antibody detects J chain in IgM only in reducing conditions. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
