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You are here:Home » Antibodies » Abs to Immunoglobulins » Anti -J Chain

Anti -J Chain

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH
J Chain serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also help to bind these immunoglobulins to secretory component.
Catalog #J1255
ApplicationsSuitable for use in Immunohistochemistry, ELISA, Immunoprecipitation and Western Blot. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (Frozen): 1:200
ELISA: 20ug/ml
Immunoprecipitation: 20ug/ml
Optimal working dilutions to be determined by researcher.
HybridomaNS1 myeloma cells with spleen cells from Balb/c mice.
Recommended Secondary AntibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-14C: IgG, Fc (HRP) Pab Gt xMo
I1904-19C: IgG, F(abí)2 (HRP) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H2560
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.0, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman J Chain.
SpecificityRecognizes human J chain as tested by ELISA using purified human Ig fragments as the solid phase target antigen. The antibody does not detect J chain in intact IgM in ELISA assays due to steric hindrance. In Western Blot techniques the antibody detects J chain in IgM only in reducing conditions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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