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You are here:Home » Molecular Biology » MB-Protein Kinases » JNK1 alpha1, active Recombinant Human (c-Jun N-terminal Kinase 1, JNK, JNK-46, Mitogen-activated Protein Kinase 8, MAP Kinase 8, MAPK 8, MAPK8, PRKM8, Stress-activated Protein Kinase 1c, SAPK1c, SAPK1, Stress-activated Protein Kinase JNK1)

JNK1 alpha1, active Recombinant Human (c-Jun N-terminal Kinase 1, JNK, JNK-46,
Mitogen-activated Protein Kinase 8, MAP Kinase 8, MAPK 8, MAPK8, PRKM8, Stress-activated
Protein Kinase 1c, SAPK1c, SAPK1, Stress-activated Protein Kinase JNK1)

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Specifications

Catalog #J2500
Recombinant full-length human JNK1a1/SAPK1c with an N-terminal His-tag, expressed in Sf21 cells. Accession Number L26318.
Specific Activity 39u/mg where one Unit of JNK1a1/SPAK1c activity is equal to 1nmol phosphate incorporated into GST-ATF2 per minute.
Storage and Stability Stable for 6 months at- 70°C from date of shipment.
JNK1a1/SAPK1c Kinase Assay 0.520mU of JNK1a1/SAPK1c phosphorylated ATF2 substrate in the assay.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 2.5ug of active JNK1a1. References 1. Gupta, S., et al., EMBO J. 15: 27602770, 1996. 2. Gupta, S., et al., Science 267: 389393, 1995. 3. Lawler, S., et al., FEBS Lett 414: 153158, 1997.
Molecular Weight43kD
SourceRecombinant human
PurityPurified to 90% using Ni-NTA agarose. Purity was assessed by SDS-PAGE and Coomassie blue staining using 2.5ug of active JNK1a1.
FormSupplied as a frozen solution in 60ul of 50mM Tris-HCl, pH 7.5, 270mM sucrose, 150mM NaCl, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF.
Intended for research use only, not for use in human, therapeutic or diagnostic applications.
Alternate namesEC=2.7.11.24


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