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You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -Junctional Adhesion Molecule 1 (JAM-1, CD321, F11 Receptor, F11R, Junctional Adhesion Molecule A, JAMA, JCAM, KAT, Platelet Adhesion Molecule 1, PAM1, Platelet F11 Receptor, UNQ264/PRO301)

Anti -Junctional Adhesion Molecule 1 (JAM-1, CD321, F11 Receptor, F11R, Junctional
Adhesion Molecule A, JAMA, JCAM, KAT, Platelet Adhesion Molecule 1, PAM1, Platelet F11
Receptor, UNQ264/PRO301)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
The junctional adhesion molecule-1 (JAM-1) is an integral membrane protein that co-localizes with tight junction molecules occludin, ZO-1, and cingulin.1 JAM-1 has been characterized as a member of the immunoglobulin (Ig) superfamily and is composed of an extracellular domain, a single-pass transmembrane region, and a short cytoplasmic tail.2 JAM-1 is localized at tight junctions in endothelial and epithelial cells and its sub-cellular distribution is modulated by inflammatory cytokines.2 JAM-1 is also expressed on circulating leukocytes and platelets as well as in lymphoid.2 Most claudins as well as JAM-1 end with the amino acid Val at their C-terminal ends, suggesting that these COOH termini directly bind to PDZ domains.3 JAM-1 appears to be a single copy gene, which is constitutively expressed on various tissues and cells, and may be involved in cell-to-cell adhesion through homophilic interaction.4 JAM-1 was detected in human intestine cell lines T84, CaCO2 and HT29, in the lung cell line A 549, and the liver cell line HepG2.5
Catalog #J8009
ApplicationsSuitable for use in Western Blotting. Other applications not tested.
Recommended DilutionWestern Blotting: 13ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to the C-terminal of human JAM-1 protein
SpecificitySpeci c for human ~3641kD JAM-1 protein. Reactivity has been con rmed with Caco-2 colorectal adenocarcinoma cell lysates.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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