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Specifications

Catalog #	J9000
Cellular Protein Preparation	Cells were lysed in modified RIPA buffer (50mM Tris-HCl, pH 7.4, 1% NP40, 0.25% sodium deoxycholate, 150mM sodium chloride, 1mM EDTA, 1mM PMSF, 1ug/ml aprotinin, 1ug/ml leupeptin, 1ug/ml pepstatin, 1mM sodium orthovanadate, 1mM sodium fluoride). Diluted with non-reducing sample buffer (31mM Tris-HCl, pH 6.8, 5% glycerol, 1% SDS, 0.002% bromophenol blue).
Applications	Suitable for use in Western Blot as a positive control for antibodies. Other applications not tested
Recommended Usage	Add 2.5ul 2-mercaptoethanol/100ul of lysate. Boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for Western Blot analysis. For use as a positive control for antibodies.
Storage and Stability	For long-term storage, aliquot and store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Concentration	~1mg/ml
Form	Supplied as a liquid in modified RIPA buffer with non-reducing sample buffer.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.