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You are here:Home » Antibodies » Antibodies-Transcription Factors, Zinc (Ring) Finger » Anti -Kaiso (Zinc Finger and BTB Domain Containing 33, ZBTB33)

Anti -Kaiso (Zinc Finger and BTB Domain Containing 33, ZBTB33)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH
Catalog #K0004
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and Supershift. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry: 1ug/ml gives positive nuclear immunostaining for Kaiso in NIH 3T3 cells fixed with 100% methanol.
Western Blot: 0.5-2ug/ml detects Kaiso in RIPA lysates from 3T3/A31 cells.
Immunoprecipitation: 2-5ug immunoprecipitates Kaiso from RIPA lysates from NIH 3T3 cells.
Supershift: Supershifts a specific methyl-CpG complex.
Optimal dilutions to be determined by the researcher.
Positive Antigen ControlF4209: 3T3/A31 cell lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2T15
HostMouse
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in TGS, pH 7.4, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant N-terminal GST and His-tagged fusion protein corresponding to the central region of murine Kaiso (aa103584).
SpecificityRecognizes mouse Kaiso, Mr 97kD. Species Crossreactivity: human, rat, canine, chicken, hamster and simian
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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