| Kaiso is a transcriptional represor that belongs to POZ-zinc finger family of transcription factors implicated in development and cancer. It binds to specific methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and it also binds to non-methylated consensus sequence 5'-CTGCNA-3'. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of represive chromatin structures in taget gene promoters. Kaiso may contribute to the repression of target genes of the Wnt signaling pathway. |
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| Catalog # | K0004 |
| Kaiso is a novel member of the BTB/POZ (Broad complex, Tramtrak, Bric à brac/Pox virus and zinc finger) zinc finger superfamily of transcription factors. Yeast 2-hybrid studies have shown that Kaiso interacts with catenin p120ctn. This interaction involves the Arm repeats of 1-7 of the p120ctn Armadillo domain and the carboxyl-terminal third of Kaiso. |
| Applications | Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and Supershift. Other applications not tested. |
| Recommended Dilution | Immunohistochemistry: 1ug/ml gives positive nuclear immunostaining for Kaiso in NIH 3T3 cells fixed with 100% methanol. |
| Western Blot: 0.5-2ug/ml detects Kaiso in RIPA lysates from 3T3/A31 cells. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Kaiso (2ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. |
| Immunoprecipitation: 2-5ug immunoprecipitates Kaiso from RIPA lysates from NIH 3T3 cells.1, 3 |
| Supershift: Supershifts a specific methyl-CpG complex. |
| Optimal dilutions to be determined by the researcher. |
| Positive Antigen Control | 3T3/A31 cell lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 2T15 |
| Host | Mouse |
| Source | Mouse |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Recombinant N-terminal GST and His-tagged fusion protein corresponding to the central region of murine Kaiso (aa 103-584). |
| Specificity | Recognizes Kaiso, Mr 97kD. Species Crossreactivity: Human, mouse, rat, canine, chicken, hamster and simian. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
