Kallikrein 9 is a secreted protein with function unknown.
Applications
Suitable for Western Blot, Immunoprecipitation and Direct ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 1ug/ml. Using a colorimetric detection system, the detection limit for recombinant human KLK9 is ~25ng/lane and 10ng/lane under non-reducing and reducing conditions, respectively. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Immunoprecipitation: 25ug/ml to immunoprecipitate recombinant human KLK9 from conditioned cell culture medium. Optimal dilution determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Asp20-Asn250 from human Kallikrein, expressed in NS0 cells. Species Sequence Homology: canine; 89%, rat; 82% and mouse; 77%
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes human KLK9. Does not crossreact with rhKLK1, -3, -4, -5, -6, -7, -8, -10, -11, -12, -13, -14, -15, rhKLKB1, rhHGFA, rhFactor VII, rhFactor X, rhFactor XI or rhThrombin.