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You are here:Home » Antibodies » Abs to Kallikrein » Anti -Kallikrein 9 (KLK9, KLK L3)

Anti -Kallikrein 9 (KLK9, KLK L3)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP
Kallikrein 9 is a secreted protein with function unknown.
Catalog #K0005-12A
ApplicationsSuitable for Western Blot, Immunoprecipitation and Direct ELISA. Other applications not tested.
Recommended DilutionsWestern Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit for recombinant human KLK9 is ~25ng/lane and 10ng/lane under non-reducing and reducing conditions, respectively. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.
Immunoprecipitation: 25ug/ml to immunoprecipitate recombinant human KLK9 from conditioned cell culture medium.
Direct ELISA: 0.5-1.0ug/ml. The detection limit for ecombinant human KLK9 is ~10ng/well.
Optimal dilutions should be determined by each laboratory for each application.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No7H142
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa20-250 from human Kallikrein expressed in NS0 cells (AAD26427). Species Sequence Homology: canine; 89%, rat; 82% and mouse; 77%
SpecificityRecognizes human KLK9. Does not crossreact with rhKLK1, -3, -4, -5, -6, -7, -8, -10, -11, -12, -13, -14, -15, rhKLKB1, rhHGFA, rhFactor VII, rhFactor X, rhFactor XI or rhThrombin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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