| Kallikrein 9 is a secreted protein with function unknown. |
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| Catalog # | K0005-12A |
| Applications | Suitable for Western Blot, Immunoprecipitation and Direct ELISA. Other applications not tested. |
| Recommended Dilutions | Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit for recombinant human KLK9 is ~25ng/lane and 10ng/lane under non-reducing and reducing conditions, respectively. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. |
| Immunoprecipitation: 25ug/ml to immunoprecipitate recombinant human KLK9 from conditioned cell culture medium. |
| Direct ELISA: 0.5-1.0ug/ml. The detection limit for ecombinant human KLK9 is ~10ng/well. |
| Optimal dilutions should be determined by each laboratory for each application. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2b |
| Clone No | 7H142 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.5mg/ml (after reconstitution) |
| Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Recombinant protein corresponding to aa20-250 from human Kallikrein expressed in NS0 cells (AAD26427). Species Sequence Homology: canine; 89%, rat; 82% and mouse; 77% |
| Specificity | Recognizes human KLK9. Does not crossreact with rhKLK1, -3, -4, -5, -6, -7, -8, -10, -11, -12, -13, -14, -15, rhKLKB1, rhHGFA, rhFactor VII, rhFactor X, rhFactor XI or rhThrombin. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
