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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IP

Kallikrein 9 is a secreted protein with function unknown.
Catalog #	K0005-12A
Applications	Suitable for Western Blot, Immunoprecipitation and Direct ELISA. Other applications not tested.
Recommended Dilutions	Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit for recombinant human KLK9 is ~25ng/lane and 10ng/lane under non-reducing and reducing conditions, respectively. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.
	Immunoprecipitation: 25ug/ml to immunoprecipitate recombinant human KLK9 from conditioned cell culture medium.
	Direct ELISA: 0.5-1.0ug/ml. The detection limit for ecombinant human KLK9 is ~10ng/well.
	Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	7H142
Host	Mouse
Source	Human
Concentration	~0.5mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Recombinant protein corresponding to aa20-250 from human Kallikrein expressed in NS0 cells (AAD26427). Species Sequence Homology: canine; 89%, rat; 82% and mouse; 77%
Specificity	Recognizes human KLK9. Does not crossreact with rhKLK1, -3, -4, -5, -6, -7, -8, -10, -11, -12, -13, -14, -15, rhKLKB1, rhHGFA, rhFactor VII, rhFactor X, rhFactor XI or rhThrombin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.