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Specifications

Kanamycin sulfate is an antibiotic that belongs to the group of drugs called aminoglycosides. It binds to ribosomal components and inhibits protein synthesis. Kanamycin sulfate appears to prevent bacteria from making their cell walls; thus the cells die. It is used to treat many sensitive gram-negative, some gram-positive bacteria and and mycoplasmas in cell culture. Kanamycin is inactivated by an aminoglycoside phospho transferase (MW 25,000) in the periplasmic space.
Catalog #	K0015
Application	Suitable for use as a selectable marker for cosmid vectors used in transfection of mammalian cells.
Form	Filter sterilized (0.2um) solution in 0.9% saline. Supplied in 20ml amber screw-cap vials.
	pH:6.5-8.5
	Appearance: Clear solution
	Chromatographic Purity: To pass USP
	Identity: To pass USP
	Activity: 750ug/mg
	Crystallinity: To pass USP
	Specific Rotation: +107° to +121°
	Endotoxin: 2Eu/mg
	Kanamycin B Content (TLC): 4%
	pH (Acidity or Alkalinity): 6.5-8.5
	Sulfate: 15.0-17.0%
	Sulfated Ash: 0.5%
	Abnormal Toxicity: To pass BP98
	Depressor Substance: To pass BP98
Working Concentration	Cell/Tissue Culture: ~30ug/ml
	Stringent Plasmids: 10ug/ml
	Relaxed Plasmids: 50ug/ml
Storage and Stability	Store liquid at 4°C. Stable for for 12 months at 4°C. Further dilutions can be made in assay buffer. Stability at 37°C: 5 days.
CAS Number	25389-94-0
Molecular Formula	C18H36N4O11•H2SO4
Molecular Weight	582.58
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	Streptomyces kanamyceticus; Kanamycin A; Kanamycin monosulfate