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You are here:Home » Biochemicals » Biochem-Antibiotics » Kanamycin Sulfate 50mg/ml Solution

Kanamycin Sulfate 50mg/ml Solution

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Specifications

Kanamycin sulfate is an antibiotic that belongs to the group of drugs called aminoglycosides. It binds to ribosomal components and inhibits protein synthesis. Kanamycin sulfate appears to prevent bacteria from making their cell walls; thus the cells die. It is used to treat many sensitive gram-negative, some gram-positive bacteria and and mycoplasmas in cell culture. Kanamycin is inactivated by an aminoglycoside phospho transferase (MW 25,000) in the periplasmic space.
Catalog #K0015
ApplicationSuitable for use as a selectable marker for cosmid vectors used in transfection of mammalian cells.
Form Filter sterilized (0.2um) solution in 0.9% saline. Supplied in 20ml amber screw-cap vials.
pH:6.5-8.5
Appearance: Clear solution
Chromatographic Purity: To pass USP
Identity: To pass USP
Activity: 750ug/mg
Crystallinity: To pass USP
Specific Rotation: +107° to +121°
Endotoxin: 2Eu/mg
Kanamycin B Content (TLC): 4%
pH (Acidity or Alkalinity): 6.5-8.5
Sulfate: 15.0-17.0%
Sulfated Ash: 0.5%
Abnormal Toxicity: To pass BP98
Depressor Substance: To pass BP98
Working ConcentrationCell/Tissue Culture: ~30ug/ml
Stringent Plasmids: 10ug/ml
Relaxed Plasmids: 50ug/ml
Storage and StabilityStore liquid at 4°C. Stable for for 12 months at 4°C. Further dilutions can be made in assay buffer. Stability at 37°C: 5 days.
CAS Number25389-94-0
Molecular FormulaC18H36N4O11H2SO4
Molecular Weight582.58
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesStreptomyces kanamyceticus; Kanamycin A; Kanamycin monosulfate


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