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Anti -KAP-1 (TRIM28, KAP1, TF1B, RNF96, TIF1B, tripartite motif-containing 28, nuclear corepressor)
Anti -KAP-1 (TRIM28, KAP1, TF1B, RNF96, TIF1B, tripartite motif-containing 28, nuclear
corepressor)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B IH IF |
|
| Catalog # | K0075-05A | | Applications | Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | ELISA: 1:8000. | | Western Blot: Approx 100-120kD band seen in HepG2 lysate. Recommended for use at 0.2-1ug/ml (Predicted MW approx 92kD according to NP_005753). | | Immunohistochemistry: 0.1ug/ml. Successfully used in IHC assays on human gastric cancer surgical specimens: Yokoe et al, Ann Surg Oncol. 2009 Nov 7. [Epub ahead of print]. (PMID: 19898899.) | | Immunofluorescence: Successfully used in on mouse embryo at the late zygote stage, 5ug/ml (personal communication). | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Goat | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | C-SSQELSGGPGDGP, C Terminus | | Specificity | Recognizes Human KAP1/TRIM28 | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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